It was strict regulation of trade under the Navigation Acts that caused the colonist to…
A. believe that they (the colonists) were so important to British mercantilism that they (the colonists) could demand further rights.
B. become aware of their true place in the empire: as suppliers and markets for England, NOT as equal trading partners with the British homeland.
C. see that the laws were so strict that any rebellion against England would be a failure.