At pH 6.0 the N-terminus of His-His-His-His would have a Entry field with correct answer positive charge. The C-terminus would have a Entry field with correct answer negative charge. Since the pKa for His is 6.0, essentially half of the residues would carry a Entry field with correct answer positive charge on their side chains, so that the charge per residue could be considered to be Entry field with incorrect answer 2.0 . The result would be a net charge on the tetrapeptide of Entry field with incorrect answer 1.0 . Partially correct answer. Your answer is partially correct. Try again. The tetrapeptide would be Entry field with correct answer less soluble in water than Histidine itself. When the_______ carbon bonds are formed between the carboxylate and amino groups to form the tetrapeptide, the groups that would interact with water are lost.