What is not true of electroporation?
a.The bacterial membranes open up temporarily to allow the negatively charged DNA to go into the bacterial cytoplasm
b.Plasmids and bacterial cells are mixed in cold solution
c.The mixture of plasmids and cells are subjected to a brief electric pulse
Positively charged calcium ions coat negatively charged phosphate groups on DNA
d.The plasmids and cells are also subjected to a heat shock step