Imagine that you are a researcher continuing to study group I intron ribozyme kinetics and that you found two novel nucleotide substrates X and Y. Nucleotide substrate X has the same Vmax and Kcat as G (guanosine) but the Km is 100-fold less. Another nucleotide substrate Y has a 10-fold lower Vmax, 10-fold lower Kcat and a 2-fold higher KM. Sketch the velocity-versus-substrate graph for the three substrates G, X, and Y. Sketch the Lineweaver-Burke plot for the three substrates. Calculate the ∆∆Go of binding for X compared to G and for Y compared to G at 37 oC. Please note that all three substrates should be represented in both graphs and that ∆∆Go should be compare X to G and X to Y