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Suppose that a thin-layer chromatography plate the solvent front migrates a distance of 14 cm, and two steroid spots move distances of (a) 6 cm and (b) 12 cm. Calculate the Rf values for each steroid.

Respuesta :

Answer:

A) [tex]RF = 0.428[/tex]

B) [tex]RF = 0.857[/tex]

Explanation:

RF value in thin-layer chromatography plate is defined as the ratio of distance moved by the steroid spot (A) to the distance migrated by solvent front(B)

Hence, mathematically

[tex]RF = \frac{A}{B}[/tex] ------Equation (A)

a) Here [tex]A = 6[/tex] cm and [tex]B = 14[/tex]cm

Substituting the given values in above equation, we get -

[tex]= \frac{6}{14} \\= 0.428\\[/tex]

b) Here [tex]A = 12[/tex] cm and [tex]B = 14[/tex]cm

Substituting the given values in above equation, we get -

[tex]= \frac{12}{14} \\= 0.857\\[/tex]