If a calibration curve of absorbance vs procaine hydrochloride concentration (mol L') is linear with the equation y = 240000x for the derivatised procaine, what is the value of the molar absorptivity coefficient (Emolar)? Assume that a cuvette with a pathlength of 3 cm was used. (1 mark) Y=240000 Emolar = _ L molo

Respuesta :

Answer:

8000 L/ mol cm is the value of the molar absorptivity coefficient.

Explanation:

The slope of the absorbance- concentration graph is equal to the product of path length and  molar absorptivity coefficient.

[tex]\frac{dA}{dc}=\epsilon l[/tex]

[tex]\frac{dA}{dc}[/tex]= Rate of change of absorbance with respect to concentration

[tex]\epsilon [/tex] = molar absorptivity coefficient

l = Path-length

Given the slope of graph of absorbance vs procaine hydrochloride concentration.

y = 240000 x (linear equation)

Path length of cuvette = 3 cm

[tex]A=240000 c[/tex]

[tex]A=\epsilon c\times l=(\epsilon l)\times c[/tex]

[tex]240000=\epsilon \times 3[/tex]

[tex]\epsilon =\frac{24000}{3}=8000[/tex]

The units of [tex]\epsilon = 8000 l/mol cm[/tex]

8000 L/ mol cm is the value of the molar absorptivity coefficient.