Suppose that you have an amino acid with three dissociated groups (a-carboxyl group, a-amino group and basic R-group), with the Pkas 3, 9, and 11.
B. What is the PI
C. What is the net charge at pH 6

Respuesta :

Answer:

B. pI=10

C. Charge 1+

Explanation:

B. You'll write your pKa values from low to high, we'll build a table to find the charges for your three posible dissociated groups:

 

         -COOH   -NH3   -R     Total charge

              0             +        +            2+

pH 3 -----------------------------------------------------

               -             +         +            1+

pH 9------------------------------------------------------

               -             0         +             0

pH 11------------------------------------------------------

               -             0          0             1-

To calculate pI we'll take the pKa values where you have total charge zero (pKa 9 and 11):

[tex]pI= \frac{9+11}{2} =10[/tex]

Our pI=10

C. considering pH 6 is between pH3 and pH9 we can assume that the net charge in that pH will be 1+.

I hope you find this information useful! Good luck!