Answer:
Explanation:
Transformation of bacteria to produce enzyme will be done using genetic engineering techniques. Cells responsible for producing enzyme will be used for extraction of DNA. Gel electrophoresis will be used to visualize the DNA after restriction by binding and locating the specific gene using specific probe. The gene will be visualized and amplified using polymerase chain reaction for making copies of the gene. Thereafter, A specific plasmid will be used which have an antibiotic resistance genetic marker and will be restricted using the same restriction enzyme which is used for the amplified gene. So gene of interest and plasmid have same ends.
later, the gene will be inserted in to the plasmid using the ligation reaction for the ligation of gene of interest in to the plasmid. So after ligation the ligated product will be transformed in to the bacteria using heat shock reaction. the Transformed bacteria are allowed to grow in the culture or media containing the antibiotic. The culture will allow only those bacteria to grow in medium which are transformed with plasmid. later the screening of the recombinant (having gene of interest) and non recombinant plasmid will be done. so bacteria with recombinant plasmid will produce the digestive enzyme.
