Respuesta :
Liquid Detergent in the Extraction Buffer- dissolves the plasma membrane and binds proteins and carbs
Salt in the Extraction Buffer -negates the negative charges in DNA
Alcohol in the Extraction Buffer - precipitates the DNA
wheat germ-source of DNA
DNases - enzymes that cut DNA
Explanation:
DNA is isolated by the purification of the sample in molecular biology.
The role of buffers is to isolate buffer in the optimum pH without degrading the DNA. Major steps involved in DNA extraction are: lysis, precipitation and purification.
Liquid detergent DNA: The fats, carbohydrate and proteins that make up the cell membrane or membrane around nucleus are broken and DNA gets release.
Salt in the Extraction Buffer: When ionic bond between cation of the salt as Na+ bonds with PO3^2 of sugar backbone of DNA it gets precipitated.
Alcohol in the Extraction Buffer : It aggregates the DNA in the form of pellet and precipitate it. It basically cleans the DNA of all unwanted impurities. This is purification step.
Wheat germ: To extract DNA from wheat germ, the cellulose cell wall is destroyed first. It is a source of DNA in isolation process.
DNases: Also called deoxyribonuclease enzyme responsible for catalyzing the hydrolytic cleavage of phosphodiester bonds of DNA.
