Answer:
22 cells
Explanation:
Given that:
The concentration of numbers of cells = 6.74 x 10⁶ cells/ml
Dilution stages includes = 1:100 ; 1:100 & 1:3
The consecutive dilution stages can be calculated as:
= [tex](6.74*10^6)*(\frac{1}{100})(\frac{1}{100})(\frac{1}{3})[/tex]
= 222.42 cells/ml
= 2.22 × 10² cells/ml
So after addition 0.1 ml of the final dilution to a spread plate, the number of CFUs ( Colony forming units) we expect to count will be:
= (2.22 × 10² cells/ml)(0.1 ml)
= 22.2 cells
≅ 22 cells
∴ the number of CFUs we expect to count on our spread plate the next day = 22 cells
