) If the following mixture of proteins was applied to a size-exclusion chromatography column, what would be the order of elution?

Proteins with molecular weights: myoglobin (17.7 kDa) , hemoglobin (64.5 kDa) , lysozyme (14.3 kDa) and triose phosphate isomerase (57.4 kDa)

a) lysozyme, myoglobin, triose phosphate isomerase, hemoglobin
b) triose phosphate isomerase, hemoglobin, lysozyme, myoglobin
c) hemoglobin, myoglobin, lysozyme, triose phosphate isomerase
d) hemoglobin, triose phosphate isomerase, myoglobin, lysozyme,
e) cannot be determined

Respuesta :

Answer: Option A

Explanation:

The size exclusion chromatography is a type of chromatography which is based on the size of the molecules.

The solution is separated based on the molecular weight and size. It is usually used the separation of the macro molecules.

The sample carrying the mixture of substances is put into the column and then based and the weight or size the smaller molecules comes first and the larger and heavier molecules comes later.

So, the order will be Option A