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A particular cell type spends 4 hours in G1 phase, 2 hours in S phase, 2 hours in G2 phase, and 30 minutes in M phase. If a pulse-chase experiment was performed with radioactive thymidine on an asynchronous culture of such cells, what percentage of mitotic cells would be radiolabeled 9 hours after the pulse

Respuesta :

Answer:

0%.

Explanation:

1. As given, cell cycle is of 8.30 hours.

G1 phase= 4 hours;

S phase= 2 hours;

G2 phase = 2 hours;

M phase= 30 minutes.

2. Adding up each phase shows that the cell cycle is 8.5 hours long. As after 9 hours, the radio labeled cells would have passed through a full cycle ( from G-1 to m phase) and be in either S phase or G2- phase and  none cell  would have entered M Phase.

3. DNA  synthesis occurs only in S-phase, so the incorporation of any radioactive nucleotide happens in S-phase only.

4. M phase, cell divide itself into two daughter cells.

Histrionicus

The percentage of mitotic cells would be radiolabeled 9 hours after the pulse - 0%.

Interphase

There are three-phase G1, S, and G2 phase. Interphase is the phase that takes a maximum time of the cell cycle.

Adding up each phase allows you to determine that the cell cycle is 8.5 hours long.

  • G1 phase will be of  4 hours
  • S phase will be of 2 hours
  • G2 phase will be of 2 hours and
  • M phase will be of 30 minutes

There would be a total cell cycle is of 8.30 hours. After 9 hours, the radiolabeled cells would have passed through a full cycle and be in either S phase or G2-

So there would be 0% of mitotic cells would have entered M Phase.

Learn more about Interphase:

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