The change in t between pH 6 and pH 7 must involve_________ of a proton in the active site. The best candidate is _______. The increase in Km at higher pH must involve a change in the binding site. The group involved is probably the ________ at Ile 16, created by the cleavage that activates chymotrypsin.
N-terminus
gain
His 57
Asp 102
C-terminus
Ser195
loss