We have that the A340 will change as a consequence of lactate dehydrogenase activity because
- A340 would decrease as pyruvate is reduced to lactate
- Option D
From the question we are told
How would A340 change as a consequence of lactate dehydrogenase activity.
Assume that the assay media contains only pyruvate and NADH at the beginning of the experiment.
Absorbtion of light
Generally as a consequence of lactate dehydrogenase activity the solution of NADH is will be converted and will give
NAD+
Thereofore
Absorbtion of light at 340nm would reduce as the NADH in solution would be converted to NAD+
Hence
A340 would decrease as pyruvate is reduced to lactate
Option D
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