Start with the temperature at 25 ºC and the pH at 7. Do not vary these parameters while testing for substrate (Initial Lactose) concentration, as they are environmental controls. Substrate (Initial Lactose) concentration is in mg/dL.
Vary the substrate (Initial Lactose) concentration by 250 mg/dL, starting with 0 mg/dL.
Click the green ‘Run’ button.
After the experiment runs, click the graph between 2 and 4 minutes and move it until the time underneath the graph shows ‘Minutes: 3’ and hold it there.

Start with the temperature at 25 ºC and the pH at 7 Do not vary these parameters while testing for substrate Initial Lactose concentration as they are environme class=