The Concenteration of a solution that has an absorbance of 0.420
is 5.27 μМ
What is Absorbance ?
Absorbance (A), also known as optical density (OD), is the quantity of light absorbed by a solution.
Transmittance is the quantity of light that passes through a solution.
We can measures the absorbance of a sample using a spectrophotometer and the value is A = 0.420.
We can calculate the concentration of the sample using the Beer-Lambert law, which relates the attenuation of light to the properties of the material through which the light is travelling, through the following equation.
A = ∈ x c x d
Where ;
- A = Absorbance
- ∈ = molar absorptivity of the species (7.96 × 10⁴ M⁻¹ cm⁻¹)
- c = concentration
- d = optical path length (1.00 cm)
Therefore,
c = A / ∈ x d
= 0.420 / 7.96 × 10⁴ M⁻¹ cm⁻¹ x 1.00 cm
= 5.27 μМ
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