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NAD+ serves as an electron acceptor in glycolysis. It accepts two electrons to become reduced to NADH + H+.
NAD+ is a limiting factor in glycolysis. In the absence of ADP, the conversion of NADH to NAD+ via the electron transport chain is prevented from happening.
In eukaryotes, the hydrogen atoms of the molecules mentioned in are transferred to certain organic molecules in one of two methods: alcohol fermentation as can occur in yeast and lactic acid fermentation as can occur in human cells. In the process mentioned in, the hydrogen atoms are passed to acetaldehyde, which is then converted to a molecule of ethanol. During this a molecule of NADH is oxidized to a molecule of NAD+, which allows glycolysis to continue on.
In conclusion, NAD+ concentration is limiting to glycolysis.
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