Your research partner has centrifuged the cell extract at 10,000g for 10 minutes, but is not sure which fraction (pellet or supernatant) contains mdh. what should you tell your research partner?

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22-07-2022
Social Studies

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Your research partner has centrifuged the cell extract at 10,000g for 10 minutes, but is not sure which fraction (pellet or supernatant) contains mdh. what should you tell your research partner?

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MubeenaD MubeenaD · Answer 1 · 2022-07-30T09:56:23+02:00

Both the pellet (mitochondrial MDH) and the supernatant (cytoplasmic MDH) contain MDH.

Separation has been achieved. One does not need to separate each fraction to separate two cell fractions from each other.

Adding additional steps before and after the mitochondrial step will increase the purity, but based on the description to isolate the two enzymes, the job is done. Tell your partner to keep both fractions.

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