Energy released by the electron transport chain is used to pump H⁺ ions in eukaryotic cells into the (C) mitochondrial intermembrane space.
Electron Transport Chain (ETC) is a series of proteins complexes embedded in the inner mitochondrial membrane, that perform the function of synthesis of ATP. This ATP synthesis occurs due to the formation of the H⁺ gradient at the intermembrane space. Transfer of electrons occurs through the protein complexes coupled with proton transfer.
Mitochondrial intermembrane space is the region between the two plasma membranes of the mitochondria. The region occupies the minimal space of the mitochondria, yet it is very crucial for the ATP synthesis to occur.
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