Around 30 - 300 colonies is considered the most appropriate for calculation of number of bacteria in the sample. Anything below 30 colonies is considered too few to count.
What is CFU?
- Accordingly, CFU/g and CFU/ml stand for colony-forming units per gram and milliliter, respectively.
- In a colony-forming unit, a single microbe produces a colony of bacteria on a petri dish.
- The term "Total Viable Count" refers to the total number of colonies (TVC).
- Additionally, CFU on a probiotic label denotes the quantity of living cells in each serving.
- They can be 2 billion to 75 billion and are typically counted in billions.
- A colony-forming unit (CFU) calculates the proportion of live microorganisms that may multiply through binary fission in a sample under controlled conditions.
- On a suitable semisolid growth medium, a CFU is defined as a single, viable propagule that yields a single colony (a population of the cells visible to the human eye).
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