Respuesta :
When axons are loaded with fura-2, an increase in cytoplasmic fluorescence is expected when the neuron is at rest.
A ratiometric and sensitive indicator dye for determining intracellular calcium is called fura-2. Since its debut in 1985, fura-2 has been referenced in several studies that discuss its uses in a range of cell types. These crucial [tex]Ca^{2+}[/tex](calcium) indicators, created by Roger Tsien and others, have significantly advanced knowledge of the function of calcium in cellular control.
An essential feature of this probe is its capacity to perform ratio measurements. The impacts of uneven dye loading, dye leakage, and photobleaching, as well as issues with measuring [tex]Ca^{2+}[/tex](calcium) in cells with variable thickness, are all significantly diminished by ratio measurements. Fura-2 fluorescence measurements may typically be done over an hour without suffering a major loss of accuracy.
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