Esther Lederberg first identified lambda in 1951 after sequencing and extracting the DNA core.
Bacteriophage is a temperate phage that enters either the lytic or lysogenic replication route after infecting Escherichia coli. The latter is distinguished by the prophage's integration into the genome of the bacterial host, where it is latently passed along to daughter cells.
For the production of DNA size marker fragments and as a substrate for restriction endonucleases, phage lambda DNA is frequently used. The mutant cI857Sam7, which carries four known mutations, is employed for mass phage DNA isolation. These mutations can be found in the DNA sequence required to create a phage alpha restriction map. Applications.
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