Proteins are built from amino acids. Proteins that contain the amino acids tryptophan, phenylalanine, and tyrosine can be quantified using UV spectroscopy and do not require a detection reagent such as Coomassie Blue. Why is this the case?

Respuesta :

Answer:

The amino acids tryptophan, phenylalanine and tyrosine are aromatic amino acids. That means they have in their structure an aromatic ring. Reagents like Coomasie Blu can react with an amino acid in an unspecific way. UV spectroscopic is a specific technic for identifying aromatic amino acids

Explanation:

Commasie Blu reagent has a Sulphate group (negative) which reacts with the positive part of the amino group. All amino acids have an amine group. It is because of that they have their name.  

On another hand, Aromatic amino acids can absorb ultraviolet light because it makes resonate the bonds pi in the aromatic ring.  

In conclusion, with Commasie Blu reagent it is possible to identify amino acids and with the UV light, you can identifying aromatic amino acids.