Suppose DNA polymerase synthesizes DNA at a rate of 1000 bases per minute in a newly discovered bacteria and that bacteria replicates 50% of their chromosome every 40 minutes. Calculate the total number of nucleotides in the bacterial chromosome.

Respuesta :

Answer:

320000 nucleotides

Explanation:

Given that:

The rate of synthesis by DNA polymerase = 1000 bases per minute

The time taken to replicate 50% of their chromosome = 40 min

Thus, the time taken for one complete round of replication = (40 × 2) min

= 80 min

The total number of nucleotides in the newly synthesized strand = 1000 pairs/min × 80 min × 4

= 320000 nucleotides

The total number of nucleotides in the bacterial chromosome is equal to 80000 bp (80 kb).

  • Bacteria are prokaryotic microorganisms whose genome consists of a single circular chromosome.

In this case, it is possible to obtain the duration of DNA replication from data:

  1. 40 minutes (min) >> 50% bacterial chromosome
  2. 40 x 2 = 80 minutes (min) >> 100% bacterial chromosome

The length of the bacterial chromosome can be easily calculated:

  1. DNA replication rate = 1000 base pairs (bp) per minute
  2. Total chromosome length: 80 x 1000 bp = 80000 base pairs (bp)

  • A kilobase (kb) is equal to 1000 base pairs (bp) of DNA, thereby in this case the bacterial genome is equal to 80 kb.

In conclusion, the total number of nucleotides in the bacterial chromosome is equal to 80000 bp (80 kb).

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